Ramona is the microscopy toolset for cutting-edge biological scientists. We unlock a new perspective on cellular-level research. Our advanced computational microscope expands field-of-view without sacrificing resolution. For the first time ever, research teams can capture dynamic biological systems synchronously, as they move across space and time. Ramona’s machine learning tools help users identify what’s important and filter out the rest so they can focus on the biology, not the microscopy. With our fully-open documentation via Python API, scientists can easily develop custom assays, integrate with lab automation equipment, and build analytics tools for their workflows. Meanwhile, our intuitive software tools allow anyone in the lab to learn the system within minutes.